Device

Part:BBa_K4170050:Design

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-30)


SUMO-LbuCas13a and crRNA targeting the miR-17-5P (extra loop design) coexpression system (all-in-one


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1453
    Illegal BglII site found at 5047
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1421
    Illegal AgeI site found at 1910
    Illegal AgeI site found at 3125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5481


Design Notes

ggf


Source

ere

References