Coding
Part:BBa_K4170001:Design
Designed by: Alexandros Giannopoulos Dimitriou Group: iGEM22_Thessaloniki_Meta (2022-09-27)
bdSUMO solubility tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 289
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The SUMO coding sequence can be fused to the N-terminus of any desired recombinant protein.
Source
This part was amplified utilizing suitable primers from pGJK_His-SUMO-LbuCas13a plasmid (addgene#172488)