Composite
Part:BBa_K4167002:Design
Designed by: Xinzichu Xie Group: iGEM22_ICJFLS (2022-10-01)
Toehold switch-amilGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pET-28a-toehold switch-amilGFP plasmid was designed to comprise some antisense sequence of miRNA let-7d-3p, constant sequence, and part sequence of miRNA let-7d-3p, which form a toehold switch, as well as the gene of marker protein amilGFP. At the presence of miRNA let-7d-3p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of amilGFP, which is easily measured.
Source
miRNA let-7d-3p comes from human, amilGFP comes from Jelly fish.