Coding

Part:BBa_K4165259

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)


GST-WWW

This composite part encodes for WWW peptide which binds to misfolded tau protein (BBa_K4165007) tagged with a GST tag (BBa_K4165070).

Usage and Biology

WWW is a synthetic peptide designed to bind to microtubule associated protein tau (MAPT) at the PHF motif which is reported to drive the accumulation of paired helical filaments form of tau resulting in tau aggregates related diseases which are mostly related to dementia. It is fused to GST tag for purification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None