Coding

Part:BBa_K4165258

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)


GST-TD28REV

This composite part encodes for TDrev28 peptid, which binds to misfolded tau protein (BBa_K4165006) tagged with a GST tag (BBa_K4165070).

Usage and Biology

Td28rev is a synthetic peptide designed to bind to microtubule associated protein tau (MAPT) at the PHF motif which is reported to drive the accumulation of paired helical filaments form of tau resulting in tau aggregates related diseases which are mostly related to dementia. It is fused to GST tag for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None