Part:BBa_K4165148

W-MINK Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.

Usage and Biology

W-MINK is a peptide (DVWMINKKRK), acquired by mirror image phage display to inhibit the fibrilization of tau which is one of the main drivers of Alzheimer’s disease and other dementia diseases. PHF* (VQIINK) is the site that derive tau aggregation. W-MINK can bind to PHF* in a mean that can disrupt the interaction between fibrils and consequently reduce the aggregates.

IC₅₀ = 1.1 μM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

W-MINK is modeled by AlphaFold2, Apptest, ITASSER and RosettaFold, best model obtained from AlphaFold2

                            Figure 1.: Predicted 3D structure of Synthetic peptide W-MINK.

Table 1: Quality assessment parameters of W-MINK model.

References

1. Seidler, P. M., Boyer, D. R., Rodriguez, J. A., Sawaya, M. R., Cascio, D., Murray, K., ... & Eisenberg, D. S. (2018). Structure-based inhibitors of tau aggregation. Nature chemistry, 10(2), 170-176.