Part:BBa_K4165144

MINK Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.

Usage and Biology

MINK is a peptide with amino acid sequence of DVQMINKKRK, it is acquired by mirror phage display to inhibit the fibrillization of tau which is one of the main drivers of Alzheimer’s disease and other dementia types. PHF* (VQIINK) is one of the sites that derives tau aggregation. MINK can bind to PHF* in a way that can disrupt the interaction between the fibrils and consequently reduce the aggregates. Its IC₅₀ for inhibition of tau aggregates were 22.6 μM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

MINK was modeled by AlphaFold2, Apptest, ITAsser and TrRosetta, and the best model obtained from Apptest ranking 6 out of 6

                            Figure 1.: Predicted 3D structure of Synthetic peptide MINK.

Table 1: Quality assessment parameters of MINK model.

References

1. Seidler, P. M., Boyer, D. R., Rodriguez, J. A., Sawaya, M. R., Cascio, D., Murray, K., ... & Eisenberg, D. S. (2018). Structure-based inhibitors of tau aggregation. Nature chemistry, 10(2), 170-176.