Coding

Part:BBa_K4164998:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-10-01)


ddRFPA1-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon of the ddRFPA1 was deleted and a flexible linker (GGGGS) was added between ddRFPA1 and ddRFPB1.


Source

This sequence is composed of BBa_K4164005 and BBa_K4164004.

References