Coding

Part:BBa_K4164027:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-10-09)


RXRα-3*GS Linker-CadC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321


Design Notes

The CadC domain was from BBa_K3425100 and a flexible linker (3*GGGGS) was added between CadC and RXRα.


Source

This sequence is composed of CadC domain (BBa_K3425100) and BBa_K4164003.

References