Coding

Part:BBa_K4164026:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


CadC-3*GS Linker-FXR.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1639


Design Notes

The CadC domain was from BBa_K3425100 and a flexible linker (3*GGGGS) was added between CadC and FXR.


Source

This sequence is composed of CadC domain (BBa_K3425100) and BBa_K4164002

References