Composite

Part:BBa_K4164015:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


Inductive expression of RXR-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1964
    Illegal NheI site found at 2232
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
    Illegal AgeI site found at 1481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836


Design Notes

We recombined BBa_K4164997 to plasmid pET-29a+ by homologous recombination.


Source

This sequence is composed of BBa_J64997, BBa_K4164995, BBa_K2556021, BBa_K4164997 and BBa_K923004.

References