Composite
Part:BBa_K4164011:Design
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-09-30)
constitutive expression of FXR-ddRFPA1 and RXR-ddRFPB1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2284
Illegal NheI site found at 2307
Illegal NheI site found at 4199 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1533
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2689
Illegal AgeI site found at 3716 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3071
Illegal SapI.rc site found at 1122
Design Notes
We recombined BBa_K4164009 and BBa_K4164010 by homologous recombination.
Source
This sequence is composed of BBa_K416009 and BBa_K416010.