Part:BBa_K4164007:Design
Ssr
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our project, we employed tobacco etch virus protease (TEV) and protein degradation tag Ssr to integrate a logic operation to control the degradation of targeted proteins (mRFP) and resolve the leakage problem. The C-terminus of targeted protein was linked with a protease cleavage site (TEVENLYFQ-protease site) and a degradation tag Ssr (AANDENYAAV) in turn by linkers. When TEV was inductively expressed, it could recognize and cleave specifically at the cleavage site, and the Ssr-tagged proteins that lost the degradation tag were no longer degraded and began to work.
Codon-optimized for E.coli and synthesized by GenScript
Source
See Zhang-2022
Zhang, Yan et al. “Design and optimization of E.coli artificial genetic circuits for detection of explosive composition 2,4-dinitrotoluene.” Biosensors & bioelectronics vol. 207 (2022): 114205. doi: 10.1016/j.bios.2022.114205