Part:BBa_K4164003:Design
Retinoid X receptor-alpha(RXRα)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 357
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 739
Design Notes
RXRs are associated with multiple cellular processes from cell proliferation to cell metabolism. RXRs have the ability to form heterodimers with subfamily 1 nuclear receptors including CAR, FXR, LXR, PPAR, PXR, RAR, TR, and VDR. As with other type II nuclear receptors, the RXR heterodimer in the absence of ligand is bound to hormone response elements complexed with corepressor protein. Binding of agonist ligands to RXR results in dissociation of corepressor and recruitment of coactivator protein, which, in turn, promotes transcription of the downstream target gene into mRNA and eventually protein.
In our project, we took advantage of the characteristics that RXR can bind to the farnesoid X receptor (FXR) as a heterodimer, using FXR as the receiver, and when activated, formed a dimer with RXR, so that the downstream ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.
Source
The retinoid X receptor (RXR) is a protein derived from humans. The gene sequence of this part was obtained from NCBI, and its GenBank Accession number is NP_002948.1.