Coding

Part:BBa_K4164002:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


farnesoid X receptor(FXR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1057


Design Notes

FXR is a protein from humans, it is a member of a member of the nuclear receptor superfamily of ligand-activated transcription factors, and functions as a receptor for bile acids. It is encoded by the NR1H4 gene in humans.

As a transcription factor, it binds to a common partner for NRs, retinoid X receptor (RXR), as a heterodimer, to regulate the expression of various genes involved in bile acid (BA), lipid, and glucose metabolisms. Both conjugated and unconjugated bile salts are able to activate FXR at physiological concentrations, the hydrophobic BA chenodeoxycholic acid (CDCA) is the most effective activator of FXR.

In our project, we took advantage of the characteristics of CDCA as the most effective activator of FXR, using FXR as the receiver, and when activated, formed a dimer with RXR, so that the downstream ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.

Source

FXR is a protein from humans, it is a member of a member of the nuclear receptor superfamily of ligand-activated transcription factors, and functions as a receptor for bile acids. The gene sequence of this part was obtained from NCBI, and its GenBank Accession number is NP_001193908.1

References