Composite

Part:BBa_K4161997:Design

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-10-10)


Usp45-signalling peptide in L.lactis-20ipaD-AcmA3b


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 591
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Appropriate linker sequences should be added between each module. Each linker is made up of two neutral amino acids.


Source

Usp45 and a fragment of L.lactis signaling peptide come from the genomic sequence of L.lactis.

References