Plasmid_Backbone

Part:BBa_K4161302:Design

Designed by: Qizhou Jiang   Group: iGEM22_DKU   (2022-07-25)


pyD1 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal NheI site found at 858
    Illegal PstI site found at 801
    Illegal PstI site found at 941
    Illegal NotI site found at 962
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal BamHI site found at 921
    Illegal XhoI site found at 969
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
    Illegal AgeI site found at 71
    Illegal AgeI site found at 1038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1020
    Illegal BsaI site found at 3646
    Illegal SapI.rc site found at 2624
    Illegal SapI.rc site found at 4728


Design Notes

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Source

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References