Composite

Part:BBa_K4161003:Design

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-07-20)


Csg surface display system on E. coli Nissle 1917


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2611
    Illegal XbaI site found at 82
    Illegal SpeI site found at 1795
    Illegal PstI site found at 13
    Illegal PstI site found at 1064
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2611
    Illegal SpeI site found at 1795
    Illegal PstI site found at 13
    Illegal PstI site found at 1064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2611
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2611
    Illegal XbaI site found at 82
    Illegal SpeI site found at 1795
    Illegal PstI site found at 13
    Illegal PstI site found at 1064
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2611
    Illegal XbaI site found at 82
    Illegal SpeI site found at 1795
    Illegal PstI site found at 13
    Illegal PstI site found at 1064
    Illegal AgeI site found at 2908
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An additional pTlpA promotor is preserved to ensure normal expression in gut environment.


Source

The original sequence that we borrow can be obtained from the plasmid pM1s3ATScsg-Etag (https://www.addgene.org/137946/)

References