Composite

Part:BBa_K4160008:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing RR120 VHH as affinity domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 454
    Illegal XbaI site found at 2060
    Illegal PstI site found at 748
    Illegal PstI site found at 1743
    Illegal PstI site found at 1904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 454
    Illegal NheI site found at 1117
    Illegal PstI site found at 748
    Illegal PstI site found at 1743
    Illegal PstI site found at 1904
    Illegal NotI site found at 2047
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 454
    Illegal BglII site found at 1221
    Illegal BglII site found at 1407
    Illegal BglII site found at 1671
    Illegal BamHI site found at 64
    Illegal XhoI site found at 631
    Illegal XhoI site found at 2054
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 454
    Illegal XbaI site found at 2060
    Illegal PstI site found at 748
    Illegal PstI site found at 1743
    Illegal PstI site found at 1904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 454
    Illegal XbaI site found at 2060
    Illegal PstI site found at 748
    Illegal PstI site found at 1743
    Illegal PstI site found at 1904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160003, and BBa_K2217015.

References