Regulatory

Part:BBa_K4160006:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


STAT promoter + CMVmin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

sequence found in pLS13 expression vector, Schukur et al., 20151

References

1 Schukur L, Geering B, Charpin-El Hamri G, Fussenegger M. Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis. Sci Transl Med. 2015;7(318). doi:10.1126/SCITRANSLMED.AAC4964/SUPPL_FILE/7-318RA201_SM.PDF