Regulatory
Part:BBa_K4160006:Design
Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel Group: iGEM22_TU-Eindhoven (2022-10-09)
STAT promoter + CMVmin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 53
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 53
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 53
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
-
Source
sequence found in pLS13 expression vector, Schukur et al., 20151
References
1 Schukur L, Geering B, Charpin-El Hamri G, Fussenegger M. Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis. Sci Transl Med. 2015;7(318). doi:10.1126/SCITRANSLMED.AAC4964/SUPPL_FILE/7-318RA201_SM.PDF