Regulatory

Part:BBa_K4158013:Experience

Designed by: Nanami Onishi, Eri Ito   Group: iGEM22_Waseda_Tokyo   (2022-09-25)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


in vitro experiments were conducted. tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR.

Fig. 1. FITC values before incubation (left) and after 150min when FITC is 5M

User Reviews

UNIQ2d4bba97cc030c34-partinfo-00000000-QINU UNIQ2d4bba97cc030c34-partinfo-00000001-QINU