Composite

Part:BBa_K4156120:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


AND gate-HlyE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 303
    Illegal BsaI.rc site found at 464


Design Notes

AND gate-HlyE uses the HlyE protein ( BBa_K4156084 ) inter-assembled with the AND gate ( BBa_K4156115 ) constructed by the team.


Source

Experimental constructed.

References