Regulatory

Part:BBa_K4156117:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


XOR gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI.rc site found at 351


Design Notes

The XOR gate is synthesized by nesting the cis-trans potential Bxb1 integrase and Tp901 integrase sites, enclosing a terminator B0015 in between. to ensure the smooth operation of the XOR gate, we tested it using only the p7 promoter. The design of this part is based on 2015, Science, Drew Endy.


Source

Experimental Constructed.

References

Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601