Composite

Part:BBa_K4156116

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


AND gate-mRFP

AND gate-mRFP consists of an AND logic gate ( BBa_K4156115 ) and a downstream RFP sequence, and tests the performance of the AND gate by detecting the red fluorescent signal.


Usage and Biology

We introduced a dual vector system in the same strain. The AND gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of AND gate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal AgeI site found at 1210
    Illegal AgeI site found at 1322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 303
    Illegal BsaI.rc site found at 464


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Categories
Parameters
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