Composite
Part:BBa_K4156107
Designed by: Zheng Huang Group: iGEM22_LZU-CHINA (2022-10-07)
pPepT-Bxb1-φ174E
pPepT-Bxb1-φ174E is a composite part that express lysis gene φ174E, constructed with hypoxia-sensing promoter pPepT and serine integrase Bxb1.
Usage and Biology
We designed pPepT-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pPepT and Bxb1 We will validate the function of this biobrick by measuring the viability of the bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 360
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 634
Illegal XhoI site found at 721 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1468
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Categories
Parameters
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