Composite

Part:BBa_K4156098

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


pCadC-Bxb1-φ174E

pCadC-Bxb1-φ174E is a complex component expressing the cleavage gene φ174E, constructed from the pH-sensitive promoter pCadC and the serine integrase Bxb1.


Usage and Biology

We designed pCadC-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate coupling the pCadC promoter to Bxb1. We will validate the function of this biobrick by measuring bacterial viability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 642
    Illegal XhoI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476


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