DNA

Part:BBa_K4156094

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


CAP binding site

The site that binds specifically to CAP is a sequence that partially overlaps with Plac at the upstream end of the promoter P1ac of the 1ac manipulator element


Usage and Biology

CAP is formed by the combination of CRP and cAMP.When CAP binds to this sequence, it enhances the transcriptional activity of RNA polymerase, resulting in a 50-fold increase in transcription. In contrast, when glucose is available for catabolism, cAMP concentration decreases, CRP cannot be activated, and structural gene expression of the 1ac manipulator element decreases.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None