DNA

Part:BBa_K4156091

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


attB Bxb1-Forward

attB TP901is is a recombination sites, which is recognized by serine integrase Bxb1.


Usage and Biology

The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. We introduce this element to control the switching of genetic logic gates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24


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Categories
Parameters
None