Coding

Part:BBa_K4156087

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-06)


Integrase TP901

TP901 is a kind of Serine recombinase.


Usage and Biology

As one of the key players in the genetic switch, it catalyzes the unidirectional inverse reaction according to the position and orientation of its specific recognition sites, attB and attP. [1] We introduce this element to control the switching of genetic logic gates. It also acts as a genetic signal amplifier

Characterization

We introduce this element to control the switching of genetic logic gates. Characterization details can be found at BBa_K415699  ; BBa_K4156100 (pCadC Promoter); BBa_K4156104  ; BBa_K4156105 (pLldR Promoter) ; BBa_K4156108  ; BBa_K4156109 (pPepT Promoter) ; BBa_K4156117  ; BBa_K4156118  ; BBa_K4156119 (XOR gate).

References

1 Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None