Regulatory

Part:BBa_K4156077

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-06)


Promoter Hyper-spank

Hyper-spank is an IPTG inducible promoter, designed to be part of Llprd operon

Usage and Biology

Hyper-spank promoter is an IPTG inducible promoter that has been designed for high expression. Gene expression under the control of the hyper-spank promoter can be induced by addition of IPTG. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR operon subunit ( BBa_K4156101 ).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None