Regulatory
Part:BBa_K415506:Design
Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk Group: iGEM10_MIT (2010-10-27)
pTRE-Tight L4R1 MammoBlock
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Increased number of TRE operator binding sites.
Source
Source: modified TRE promoter.