Composite

Part:BBa_K4152204:Design

Designed by: Xiaoyan Yue   Group: iGEM22_TJUSLS_China   (2022-09-29)


XhoⅠ+Linker a+propeptide+PK_MT4+His-Tag+Terminator+EcoRⅠ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1159
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1159
    Illegal XhoI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed our primers which contained restriction sites(XhoⅠ and EcoRⅠ) to insert our gene into pPIC9 and expressed it in Pichia Pastoris.


Source

NCBI

References