Composite

Part:BBa_K4152108:Design

Designed by: Xiaoyan Yue   Group: iGEM22_TJUSLS_China   (2022-09-28)


AOX1 promoter+α-factor secretion signal+propeptide+PK_MT7+His-Tag+AOX1 Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 1725
    Illegal PstI site found at 970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2349
    Illegal PstI site found at 970
    Illegal NotI site found at 2361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2349
    Illegal BamHI site found at 937
    Illegal XhoI site found at 1191
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 1725
    Illegal PstI site found at 970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 1725
    Illegal PstI site found at 970
    Illegal AgeI site found at 2427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet our research needs，we added five components to the design：AOX1 promoter, α-factor secretion signal, Propeptide, the His-tag and AOX1 terminator.


Source

We make multiple site mutations on the basis of a serine protease proteinase K from Tritirachium album limber. (PDB ID: 1ic6)

References