Composite
Part:BBa_K4152106:Design
Designed by: Qinting Lin Group: iGEM22_TJUSLS_China (2022-09-28)
AOX1 promoter+α-factor secretion signal+propeptide+PK_MT5+His-Tag+AOX1 Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2349
Illegal PstI site found at 970
Illegal NotI site found at 2361 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2349
Illegal BamHI site found at 937
Illegal XhoI site found at 1191 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2349
Illegal XbaI site found at 1725
Illegal PstI site found at 970
Illegal AgeI site found at 2427 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to meet our research needs,we added five components to the design:AOX1 promoter, α-factor secretion signal, Propeptide, the His-tag and AOX1 terminator.
Source
NCBI.