Coding

Part:BBa_K4152105:Design

Designed by: Qinting Lin   Group: iGEM22_TJUSLS_China   (2022-09-28)


5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1725
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1504
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet our research needs，we added five components to the design：AOX1 promoter, α-factor secretion signal, Propeptide, our target protein WT, the His-tag, and AOX1 terminator.


Source

We make multiple site mutations on the basis of a serine protease proteinase K from Tritirachium album limber. (PDB ID: 1ic6)

References