Coding

Part:BBa_K415202:Design

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)


cymR Repressor Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
    Illegal XhoI site found at 138
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 40


Design Notes

This repressor required site-directed mutagenesis of nucleotide 132 to a G in order to remove a PstI cut site.



Source

CymR was derived from the organism Pseudomonas Putida. The copy of cymR used in the construction of BBa_K415202 was generously donated by Noah Davidsohn.

References