Regulatory

Part:BBa_K415200:Design

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)


Strong Constitutive T5 Promoter with cmt Operator (T5-cmt)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter contains the T5 partial promoter, the sequence of which was derived from the 2010 Choi, et al. paper. Like T7 bacteriophage promoters, this T5 promoter is constitutive and strong; unlike T7 promoters, however, it does not require T7 RNA Polymerase. This means that the T5 promoter could have wide applicability as a strong promoter in Escherichia coli and other organisms.


Source

This part was synthesized via minigene construction by Mr. Gene; its source is the 2010 paper by Choi, et al., titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."

References