Coding

Part:BBa_K4152002:Design

Designed by: Junteng Wu   Group: iGEM22_TJUSLS_China   (2022-09-28)


PK_MT2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To improve the stability of wild-type proteinase K, we designed 7 mutation sites: D260N-N257C-P18Q-S173W-S197F-T16C-T20D.


Source

We make multiple site mutations on the basis of a serine protease proteinase K from Tritirachium album limber. (PDB ID: 1ic6)

References