Composite
Part:BBa_K415152:Experience
Designed by: Jason Stevens Group: iGEM10_MIT (2010-10-24)
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Applications of BBa_K415152
Assessing p8-GR2 incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415152, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody.
There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.
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