Protein_Domain

Part:BBa_K4151017:Design

Designed by: JIUN-JEN, TSAI   Group: iGEM22_CSMU_Taiwan   (2022-09-14)


rtTA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
    Illegal NgoMIV site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You need to place a promoter before rtTA to drive the Tet-on system.


Source

Escherichia coli

References