Regulatory

Part:BBa_K4149071

Designed by: Yusang Zhou   Group: iGEM22_Jiangnan-China   (2022-09-30)


ACAty_RS09855 promoter of Acidithiobacillus caldus

It is a promoter from Acidithiobacillus caldus , which we used to link the egfp to measure the promoter intensity by measuring the relative fluorescence intensity for subsequent use as a modeling training set.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 28
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


513.jpg Lxs.png

Description: We defined a promoter screening method to measure the expression intensity of different promoters. ACAty_RS09855 showed a perfect promoter intensity among all 200 promoters we tested.

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Parameters
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