Composite

Part:BBa_K4146505:Design

Designed by: Xia Wei   Group: iGEM22_BUCT   (2022-09-16)


pet-lacI-lac-aro10-adh6-T1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal BglII site found at 1541
    Illegal XhoI site found at 2371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
    Illegal NgoMIV site found at 1418
    Illegal NgoMIV site found at 6107
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

used for coding alcoholdehydrogenase and phenylpyruvate decarboxylase


Source

References

Wang P, Yang X, Lin B, Huang J, Tao Y. Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metab Eng. 2017 Nov;44:143-149. doi: 10.1016/j.ymben.2017.09.013. Epub 2017 Sep 22. PMID: 28951189.