Composite
Part:BBa_K4146505:Design
Designed by: Xia Wei Group: iGEM22_BUCT (2022-09-16)
pet-lacI-lac-aro10-adh6-T1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal BglII site found at 1541
Illegal XhoI site found at 2371 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823
Illegal NgoMIV site found at 1418
Illegal NgoMIV site found at 6107 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
used for coding alcoholdehydrogenase and phenylpyruvate decarboxylase
Source
References
Wang P, Yang X, Lin B, Huang J, Tao Y. Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metab Eng. 2017 Nov;44:143-149. doi: 10.1016/j.ymben.2017.09.013. Epub 2017 Sep 22. PMID: 28951189.