Coding
TcAnCS1

Part:BBa_K4146102:Design

Designed by: Yazhao Liu   Group: iGEM22_BUCT   (2022-09-16)


A gene to facilitate the synthesis of caffeine in engineered strains


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No special precautions.


Source

The promoter pLlacO1 was derived from BBa_K3875027. The start codon RBS is from BBa_B0034. The terminator T1 is from BBa_B0015.CS stands for caffeine synthase, and the TcAncCS1 ancestral gene is derived from Cocobroma. The duplication of TcAncCS1 at node O leads to TcAncCS2.

References