Composite

Part:BBa_K4144043:Design

Designed by: Pi Ruiqi   Group: iGEM22_WHU-China   (2022-10-11)


LacI with LVA-tag controlled by a constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The distance between RBS and lacI start codon is adjusted to guarantee the correct expression of the protein.


Source

The coding sequence of the LacI protein comes from BBa_C0012 and have been developed into a lactose-tolerated one (BBa_K4144041) after directed evolution conducted by our project this year.

References