Coding

Part:BBa_K4144041:Design

Designed by: Pi Ruiqi   Group: iGEM22_WHU-China   (2022-10-11)


Impreoved LacI protein responsive to high-level lactose only


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to maintain the normal functioning of our oscillator "Repressilator", we need LacI to tolerate relatively low-level lactose in intestine, so that it could only be activated in higher concentration provided artificially. Besides, out of operation accessibility consideration, we integrated three reporter genes into one plasmid, aiming to separate the ideal variant through conditionally selective culture.


Source

Original sequence refers to BBa_C0012, after directed evolution we develop it into a better lactose-tolerated one.

References