Coding
Part:BBa_K4144005:Design
Designed by: Boshuo Zhou Group: iGEM22_WHU-China (2022-10-02)
opMistic is a peptide from B. subtilis that can facilitate fused protein to localize membrane
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This peptide is a facilitated peptide to help protein localize into membrane. So it should be fused to terminus of recombined proteins.
Source
The primary gene sequence is gained from BBa_K2622006, and then it is optimized to be opMistic and then sent to synthesize.