Regulatory

Part:BBa_K414008:Design

Designed by: Elaine Bersaba   Group: iGEM10_Nevada   (2010-10-26)


rd29A Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


Design Notes

The rd29A promoter was isolated by the use of the rd29A designed primers in PCR. This was blunt end Topo cloned, digested with EcoR 1 and Pst 1 sites, and was then ligated to the pSB1C3 vector.


Source

This promoter came from the synthetic design that contained the rd29A Promoter + Strong Plant Kozak (RBS) + RFP in the pMA vector.

References