Composite

Part:BBa_K414006:Design

Designed by: Matthew Polasko   Group: iGEM10_Nevada   (2010-10-18)


35S Promoter + (Strong Plant RBS + GFP) From K414001


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191
    Illegal BsaI.rc site found at 1699


Design Notes

No additional design considerations. Standard part assembly protocol followed.


Source

This part was made with the 35S from BBa_K414002 (Wheeler, 2010) who obtained it from pK7FWG2 plasmid. Also the GFP with strong plant RBS was taken from BBa_K414001 (Bersaba, 2010).

References