Reporter

Part:BBa_K414004:Design

Designed by: Matthew Polasko   Group: iGEM10_Nevada   (2010-09-28)


Strong Plant RBS (Kozak sequence) + EYFP from E0030


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One consideration we made was the strength of the RBS we wanted upstream of the reporter. Evidence can be found in the literature that a spectrum of translational activity can be generated by tampering with the Kozak sequence in plants. Future teams may wish to investigate how they can tamper with their Kozak of designing a system that requires discrete but relative expression of genes initiated by the same promoters.


Source

The source of EYFP was derived using modified primers containing the Kozak sequence designed to bind to the iGEM part BBa_E0430 (Conboy,2004)

References