Part:BBa_K4139019:Design
Rec. CP-MAF
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 85
Illegal PstI site found at 343 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 85
Illegal PstI site found at 343 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 417
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 85
Illegal PstI site found at 343 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 85
Illegal PstI site found at 343 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
A chimeric protein designed to interact with PbEL04 which was put in the backbone of a mouse antibody.
Source
Mus musculus
References
Feng, J., Hwang, R. U., Hwang, S. F., Strelkov, S. E., Gossen, B. D., Zhou, Q. X., & Peng, G. (2010). Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination. Molecular Plant Pathology, 11(4), 503–512. https://doi.org/10.1111/j.1364-3703.2010.00623.x
Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System in Health and Disease. 5th edition. New York: Garland Science; 2001. The structure of a typical antibody molecule.
Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1
Lowe, D. G., Ricketts, M., Levinson, A. D., & Goeddelt, D. V. (1988). Chimeric proteins define variable and essential regions of Ha-ras-encoded protein (guanine nucleotide-binding protein/Ha-ras p21/R-ras p23/mammalian transformation). In Proc. Nail. Acad. Sci. USA (Vol. 85).